Influence of salinity on the growth and biochemical composition of the cyanobacterium Synechococcus sp.
N. Rosales, J. Ortega, R. Mora, E. Morales
Abstract
El estudio de cianobacterias aisladas de ambientes hipersalinos es de interés debido a su versatilidad metabólica y ecofisiológica para adaptarse a condiciones extremas de salinidad, temperatura, irradiancia y limitación de nutrientes. Se reporta el efecto de la salinidad a 0â?°, 35â?°, 70â?° y 100â?° sobre el crecimiento, masa seca y producción de pigmentos, proteÃnas, carbohidratos y lÃpidos de la cianobacteria Synechococcus sp. Los bioensayos fueron mantenidos con medio ALGAL equivalente a 8 mM NaNO3, aireación constante, fotoperiodo 12:12 h, 28 ± 2ºC y 156 µmol quanta mâ??2 sâ??1 de irradiancia. La cianobacteria fue capaz de crecer bajo todas las salinidades probadas. La densidad celular fue mejorada a 35â?°, con 607.64 ± 14.35 cél mLâ??1. A 100â?° se alcanzaron los máximos valores de masa seca, clorofila a, β-caroteno, zeaxantina, proteÃnas y carbohidratos con 3.87 ± 0.03 ng célâ??1; 41.86 ± 0.39 fg célâ??1; 9.03 ± 0.15 fg célâ??1; 9.74 ± 0.24 fg célâ??1; 1.95 ± 0.05 y 1.80 ± 0.05 pg célâ??1, respectivamente. Sin embargo, el mayor contenido de lÃpidos fue alcanzado a 0â?° con 0.45 ± 0.04 pg célâ??1. Esta cepa halotolerante de la cianobacteria Synechococcus muestra capacidad para modular la producción de biomasa enriquecida con pigmentos, proteÃnas, carbohidratos y lÃpidos en función de la salinidad.
The study of cyanobacteria isolated from hypersaline environments is of interest because of their metabolic and
ecophysiologic versatility in adapting to extreme conditions of salinity, temperature, irradiance and nutrient availability. The effect of salinity at 0â?°, 35â?°, 70â?° and 100â?° on the growth, dry weight, and pigment, protein, carbohydrate and lipid production of the cyanobacterium Synechococcus sp. was determined. Bioassays were kept in ALGAL medium equivalent to 8 mM NaNO3, constant aeration, 12:12 h photoperiod, 28 ± 2ºC and 156 µmol quanta mâ??2 sâ??1 of irradiance. The cyanobacterium was able to grow under all salinities tested. Cell density was optimized at 35â?°, with 607.64 ± 14.35 cells mLâ??1. The highest values of dry weight (3.87 ± 0.03 ng cellâ??1), chlorophyll a (41.86 ± 0.39 fg cellâ??1), β-carotene (9.03 ± 0.15 fg cellâ??1), zeaxanthin (9.74 ± 0.24 fg cellâ??1), proteins (1.95 ± 0.05 pg cellâ??1) and carbohydrates (1.80 ± 0.05 pg cellâ??1) were obtained at 100â?°; however, the highest lipid content (0.45 ± 0.04 pg cellâ??1) was reached at 0â?°. This Synechococcus strain shows halotolerance and the capacity to modulate the production of enriched biomass with pigments, proteins, carbohydrates and lipids in terms of salinity.
The study of cyanobacteria isolated from hypersaline environments is of interest because of their metabolic and
ecophysiologic versatility in adapting to extreme conditions of salinity, temperature, irradiance and nutrient availability. The effect of salinity at 0â?°, 35â?°, 70â?° and 100â?° on the growth, dry weight, and pigment, protein, carbohydrate and lipid production of the cyanobacterium Synechococcus sp. was determined. Bioassays were kept in ALGAL medium equivalent to 8 mM NaNO3, constant aeration, 12:12 h photoperiod, 28 ± 2ºC and 156 µmol quanta mâ??2 sâ??1 of irradiance. The cyanobacterium was able to grow under all salinities tested. Cell density was optimized at 35â?°, with 607.64 ± 14.35 cells mLâ??1. The highest values of dry weight (3.87 ± 0.03 ng cellâ??1), chlorophyll a (41.86 ± 0.39 fg cellâ??1), β-carotene (9.03 ± 0.15 fg cellâ??1), zeaxanthin (9.74 ± 0.24 fg cellâ??1), proteins (1.95 ± 0.05 pg cellâ??1) and carbohydrates (1.80 ± 0.05 pg cellâ??1) were obtained at 100â?°; however, the highest lipid content (0.45 ± 0.04 pg cellâ??1) was reached at 0â?°. This Synechococcus strain shows halotolerance and the capacity to modulate the production of enriched biomass with pigments, proteins, carbohydrates and lipids in terms of salinity.
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